Microarray analysis of DNA damage repair gene expression profiles in cervical cancer cells radioresistant to 252Cf neutron and X-rays
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Authors
Qing, Yi
Yang, Xue-Qin
Zhong, Zhao-Yang
Lei, Xin
Xie, Jia-Yin
Li, Meng-Xia
Xiang, De-Bing
Li, Zeng-Peng
Yang, Zhen-Zhou
Wang, Ge
Issue Date
2010-02-25
Type
Article
Language
en_US
Keywords
Microarray Analysis , DNA Damage Repair Gene , Cervical Cancer Cells , Radioresistant to 252Cf Neutron , X-rays
Alternative Title
Abstract
Background:
The aim of the study was to obtain stable radioresistant sub-lines from the human cervical cancer cell line HeLa by prolonged exposure to 252Cf neutron and X-rays. Radioresistance mechanisms were investigated in the resulting cells using microarray analysis of DNA damage repair genes.
Methods:
HeLa cells were treated with fractionated 252Cf neutron and X-rays, with a cumulative dose of 75 Gy each, over 8 months, yielding the sub-lines HeLaNR and HeLaXR. Radioresistant characteristics were detected by clone formation assay, ultrastructural observations, cell doubling time, cell cycle distribution, and apoptosis assay. Gene expression patterns of the radioresistant sub-lines were studied through microarray analysis and verified by Western blotting and real-time PCR.
Results:
The radioresistant sub-lines HeLaNR and HeLaXR were more radioresisitant to 252Cf neutron and X-rays than parental HeLa cells by detecting their radioresistant characteristics, respectively. Compared to HeLa cells, the expression of 24 genes was significantly altered by at least 2-fold in HeLaNR cells. Of these, 19 genes were up-regulated and 5 down-regulated. In HeLaXR cells, 41 genes were significantly altered by at least 2-fold; 38 genes were up-regulated and 3 down-regulated.
Conclusions:
Chronic exposure of cells to ionizing radiation induces adaptive responses that enhance tolerance of ionizing radiation and allow investigations of cellular radioresistance mechanisms. The insights gained into the molecular mechanisms activated by these "radioresistance" genes will lead to new therapeutic targets for cervical cancer.
Description
Citation
Qing, Y., Yang, X. Q., Zhong, Z. Y., Lei, X., Xie, J. Y., Li, M. X., Xiang, D. B., Li, Z. P., Yang, Z. Z., Wang, G., & Wang, D. (2010). Microarray analysis of DNA damage repair gene expression profiles in cervical cancer cells radioresistant to 252Cf neutron and X-rays. BMC cancer, 10, 71. https://doi.org/10.1186/1471-2407-10-71
Publisher
BMC Cancer