Extracellular vesicles mediated proinflammatory macrophage phenotype induced by radiotherapy in cervical cancer
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Authors
Ren, Junli
Li, Lili
Yu, Baofeng
Xu, Enwei
Sun, Naiping
Li, Xiaoning
Xing, Zihan
Han, Xiaodong
Cui, Yaqin
Wang, Xiaoyan
Issue Date
2022-01-21
Type
Article
Language
en_US
Keywords
Extracellular Vesicle , Macrophage , Radiotherapy , Cervical Cancer
Alternative Title
Abstract
Background:
Radiotherapy is a highly effective treatment for cervical cancer. Recent studies focused on the radiotherapy induced anti-tumor immunity. Whether tumor-derived extracellular vesicles (EVs) play roles in radiotherapy induced tumor associated macrophage (TAM) polarization remains unclear.
Materials and Methods:
This study analysed the phenotype of macrophages in cancer tissue and peripheral blood of cervical cancer patients using flow cytometry analysis. The role of EVs from plasma of post-irradiated patients on M2-like transformed macrophages was assessed. The M1- and M2-like macrophages were assessed by expression of cell surface markers (CCR7, CD163) and intracellular cytokines (IL-10, TNFα and iNOS). The capacity of phagocytosis was assessed by PD-1 expression and phagocytosis of pHrodo Red E. coli bioparticles.
Results:
Our results demonstrated that radiotherapy of cervical cancer induced an increase in the number of TAMs and a change in their subtype from the M2-like to the M1-like phenotype (increased expression of CCR7 and decreased expression of CD163). The EVs from plasma of post-irradiated patients facilitated the M2-like to the M1-like phenotype transition (increased expression of CCR7, TNFα and iNOS, and decreased expression of CD163 and IL-10) and increased capacity of phagocytosis (decreased PD-1 expression and increased phagocytosis of pHrodo Red E. coli bioparticles).
Conclusions:
Our data demonstrated that irradiation in cervical cancer patients facilitated a proinflammatory macrophage phenotype which could eventually able to mediate anti-tumor immune responses. Our findings highlight the importance of EV in the crosstalk of tumor cells and TAM upon irradiation, which potentially leading to an increased inflammatory response to cancer lesions.
Description
Citation
Ren, J., Li, L., Yu, B., Xu, E., Sun, N., Li, X., Xing, Z., Han, X., Cui, Y., Wang, X., Zhang, X., & Wang, G. (2022). Extracellular vesicles mediated proinflammatory macrophage phenotype induced by radiotherapy in cervical cancer. BMC cancer, 22(1), 88. https://doi.org/10.1186/s12885-022-09194-z
Publisher
BMC Cancer