miR-218-5p/RUNX2 axis positively regulates proliferation and is associated with poor prognosis in cervical cancer
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Authors
Rosa, Merlin Itsel Cruz-De la
Jiménez-Wences, Hilda
Alarcón-Millán, Judit
Romero-López, Manuel Joaquín
Castañón-Sánchez, Carlos Alberto
Salmerón-Bárcenas, Eric Genaro
Fernández-Tilapa, Gloria
Issue Date
2022-06-23
Type
Article
Language
en_US
Keywords
Cervical Cancer , miR-218-5p , RUNX2 , C-33A , CaSki
Alternative Title
Abstract
The overexpression of miR-218-5p in cervical cancer (CC) cell lines decreases migration, invasion and proliferation. The objective was to identify target genes of miR-218-5p and the signaling pathways and cellular processes that they regulate. The relationship between the expression of miR-218-5p and RUNX2 and overall survival in CC as well as the effect of the exogenous overexpression of miR-218-5p on the level of RUNX2 were analyzed. The target gene prediction of miR-218-5p was performed in TargetScan, miRTarBase and miRDB. Predicted target genes were subjected to gene ontology (GO) and pathway enrichment analysis using the Kyoto Encyclopaedia of Genes and Genomes (KEGG). The miR-218-5p mimetic was transfected into C-33A and CaSki cells, and the miR-218-5p and RUNX2 levels were determined by RT–qPCR. Of the 118 predicted targets for miR-218-5p, 86 are involved in protein binding, and 10, including RUNX2, are involved in the upregulation of proliferation. Low miR-218-5p expression and a high level of RUNX2 are related to poor prognosis in CC. miR-218-5p overexpression is related to decreased RUNX2 expression in C-33A and CaSki cells. miR-218-5p may regulate RUNX2, and both molecules may be prognostic markers in CC.
Description
Citation
Cruz-De la Rosa, M. I., Jiménez-Wences, H., Alarcón-Millán, J., Romero-López, M. J., Castañón-Sánchez, C. A., Salmerón-Bárcenas, E. G., & Fernández-Tilapa, G. (2022). miR-218-5p/RUNX2 Axis Positively Regulates Proliferation and Is Associated with Poor Prognosis in Cervical Cancer. International journal of molecular sciences, 23(13), 6993. https://doi.org/10.3390/ijms23136993
Publisher
International Journal of Molecular Sciences