Calreticulin upregulation in cervical cancer tissues from patients after 10 GY radiation therapy

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dc.contributor.authorOkada, Kohei
dc.contributor.authorSato, Hiro
dc.contributor.authorKumazawa, Takuya
dc.contributor.authorMori, Yasumasa
dc.contributor.authorPermata, Tiara Bunga Mayang
dc.contributor.authorUchihara, Yuki
dc.contributor.authorNoda, Shin-ei
dc.contributor.authorSuzuki, Keiji
dc.contributor.authorIkota, Hayato
dc.contributor.authorYokoo, Hideaki
dc.contributor.authorGondhowiardjo, Soehartati
dc.contributor.authorNakano, Takashi
dc.contributor.authorOhno, Tatsuya
dc.contributor.authorShibata, Atsushi
dc.date.accessioned2023-02-20T15:01:25Z
dc.date.available2023-02-20T15:01:25Z
dc.date.issued2022-12-27
dc.description.abstractPurpose: Understanding the immune response during radiation therapy (RT) in a clinical setting is imperative for maximizing the efficacy of combined RT and immunotherapy. Calreticulin, a major damage-associated molecular pattern that is exposed on the cell surface after RT, is presumed to be associated with the tumor-specific immune response. Here, we examined changes in calreticulin expression in clinical specimens obtained before and during RT and analyzed its relationship with the density of CD8+ T cells in the same patient set. Methods and Materials: This retrospective analysis evaluated 67 patients with cervical squamous cell carcinoma who were treated with definitive RT. Tumor biopsy specimens were collected before RT and after 10 Gy irradiation. Calreticulin expression in tumor cells was evaluated via immunohistochemical staining. Subsequently, the patients were divided into 2 groups according to the level of calreticulin expression, and the clinical outcomes were compared. Finally, the correlation between calreticulin levels and density of stromal CD8+ T cells was evaluated. Results: The calreticulin expression significantly increased after 10 Gy (82% of patients showed an increase; P < .01). Patients with increased calreticulin levels tended to show better progression-free survival, but this was not statistically significant (P = .09). In patients with high expression of calreticulin, a positive trend was observed between calreticulin and CD8+ T cell density, but the association was not statistically significant (P = .06). Conclusions: Calreticulin expression increased after 10 Gy irradiation in tissue biopsies of patients with cervical cancer. Higher calreticulin expression levels are potentially associated with better progression-free survival and greater T cell positivity, but there was no statistically significant relationship between calreticulin upregulation and clinical outcomes or CD8+ T cell density. Further analysis will be required to clarify mechanisms underlying the immune response to RT and to optimize the RT and immunotherapy combination approach.en_US
dc.identifier.citationOkada, K., Sato, H., Kumazawa, T., Mori, Y., Permata, T. B. M., Uchihara, Y., Noda, S. E., Suzuki, K., Ikota, H., Yokoo, H., Gondhowiardjo, S., Nakano, T., Ohno, T., & Shibata, A. (2022). Calreticulin Upregulation in Cervical Cancer Tissues From Patients After 10 Gy Radiation Therapy. Advances in radiation oncology, 8(3), 101159. https://doi.org/10.1016/j.adro.2022.101159en_US
dc.identifier.otherDOI: 10.1016/j.adro.2022.101159
dc.identifier.urihttps://hdl.handle.net/20.500.14041/5747
dc.language.isoen_USen_US
dc.publisherAdvances in Radiation Oncologyen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.subjectCalreticulin Upregulationen_US
dc.subjectCervical Cancer Tissuesen_US
dc.subjectGy Radiation Therapyen_US
dc.titleCalreticulin upregulation in cervical cancer tissues from patients after 10 GY radiation therapyen_US
dc.typeArticleen_US
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