Gene transfection efficacy assessment of human cervical cancer cells using dual-mode fluorescence microendoscopy
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Authors
Cha, Jaepyeong
Zhang, Jing
Gurbani, Saumya
Cheon, Gyeong Woo
Li, Min
Kang, Jin U.
Issue Date
2012-12-18
Type
Article
Language
en_US
Keywords
Gene Transfection Efficacy Assessment , Human Cervical Cancer Cells , Fluorescence Microendoscopy
Alternative Title
Abstract
We report a novel approach to quantitatively assess gene transfection efficacy using dual-modality microendoscopy that can simultaneously monitor both laser scanning reflectance and fluorescence imaging. The system uses a 500-μm-diameter coherent fiber bundle and permits 3.5-μm lateral resolution. Both reflectance and fluorescence images obtained from two silicon avalanche photodetectors are displaying at 1 Hz and processed automatically to calculate gene transfection efficiency (the ratio of fluorescent cells among the total cells). To validate the system performance we examined the expression of cyan fluorescent protein using human cervical cancer cells (HeLa) in four commercially available reagents. The result was compared with that using a high-resolution bench-top microscope.
Description
Citation
Cha, J., Zhang, J., Gurbani, S., Cheon, G. W., Li, M., & Kang, J. U. (2013). Gene transfection efficacy assessment of human cervical cancer cells using dual-mode fluorescence microendoscopy. Biomedical optics express, 4(1), 151–159. https://doi.org/10.1364/BOE.4.000151
Publisher
Biomedical Optics Express