A novel radioresistant mechanism of galectin-1 mediated by H-Ras-dependent pathways in cervical cancer cells
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Authors
Huang, E-Y
Chen, Y-F
Chen, Y-M
Lin, I-H
Wang, C-C
Su, W-H
Chuang, P-C
Yang, K-D
Issue Date
2012-01-12
Type
Article
Language
en_US
Keywords
Galectin-1 , Cervical Cancer , Radiosensitivity , Radioresistance , H-Ras
Alternative Title
Abstract
Galectin-1 is a lectin recognized by galactoside-containing glycoproteins, and is involved in cancer progression and metastasis. The role of galectin-1 in radiosensitivity has not previously been investigated. Therefore, this study tests whether galectin-1 is involved in the radiosensitivity mediated by the H-Ras signaling pathway using cervical carcinoma cell lines. A knockdown of galectin-1 expression in HeLa cells decreased clonogenic survival following irradiation. The clonogenic survival increased in both HeLa and C33A cells with galectin-1 overexpression. The overexpression or knockdown of galectin-1 did not alter radiosensitivity, whereas H-Ras was silenced in both cell lines. Whereas K-Ras was knocked down, galectin-1 restored the radiosensitivity in HeLa cells and C33A cells. The knockdown of galectin-1 increased the high-dose radiation-induced cell death of HeLa cells transfected by constitutively active H-Ras. The knockdown of galectin-1 inhibited the radiation-induced phosphorylation of Raf-1 and ERK in HeLa cells. Overexpression of galectin-1 enhanced the phosphorylation of Raf-1 and ERK in C33A cells following irradiation. Galectin-1 decreased the DNA damage detected using comet assay and γ-H2AX in both cells following irradiation. These findings suggest that galectin-1 mediates radioresistance through the H-Ras-dependent pathway involved in DNA damage repair.
Description
Citation
Huang, E. Y., Chen, Y. F., Chen, Y. M., Lin, I. H., Wang, C. C., Su, W. H., Chuang, P. C., & Yang, K. D. (2012). A novel radioresistant mechanism of galectin-1 mediated by H-Ras-dependent pathways in cervical cancer cells. Cell death & disease, 3(1), e251. https://doi.org/10.1038/cddis.2011.120
Publisher
Cell Death & Disease