Improving human papillomavirus (HPV) testing in the cervical cancer elimination era: the 2021 HPV LabNet international proficiency study
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Authors
Mühr, Laila Sara Arroyo
Eklund, Carina
Lagheden, Camilla
Forslund, Ola
Robertsson, Karin Dahlin
Dillner, Joakim
Issue Date
2022-09
Type
Article
Language
en_US
Keywords
Human Papillomavirus , Quality Assurance , International Standards , Cervical Cancer , Cancer Eradication
Alternative Title
Abstract
Background:
Proficient Human Papillomavirus (HPV) genotyping services are essential to support HPV and cervical cancer elimination strategies, in particular to support HPV vaccine research.
Objectives:
To perform a global HPV genotyping proficiency study, with evaluation in relation to previous proficiency studies.
Study design:
The proficiency panel contained 44 coded samples (40 samples containing one or more purified HPV types (HPV6/11/16/18/31/33/35/39/45/51/52/56/58/59/68a/68b) in human DNA, 1 human DNA control and 3 DNA extraction controls). Proficiency required detection of both single and multiple infections of 50 International Units of HPV 16/18, of 500 genome equivalents for other HPV types and no false positivity.
Results:
One hundred and thirty-two laboratories submitted 211 datasets. Most assays used (182/211 datasets) were commercially available. An all-time high of 75% of the datasets were 100% proficient. One or more false positives were found in 17.5% of datasets. Among laboratories who participated in the 2019 proficiency study, full proficiency increased from 25% in 2019 to 60% in 2021. The high overall proficiency was mostly attributable to a large number of new laboratories, which used similar assays.
Conclusions:
The worldwide deterioration in comparability and reliability of HPV testing found in 2019 is now reversed and an overall increase in proficiency is found.
Description
Citation
Arroyo Mühr, L. S., Eklund, C., Lagheden, C., Forslund, O., Robertsson, K. D., & Dillner, J. (2022). Improving human papillomavirus (HPV) testing in the cervical cancer elimination era: The 2021 HPV LabNet international proficiency study. Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 154, 105237. https://doi.org/10.1016/j.jcv.2022.105237
Publisher
Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology