Paris saponin VII suppressed the growth of human cervical cancer Hela cells
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Authors
Zhang, Wenjie
Zhang, Dian
Ma, Xi
Liu, Zhaoyang
Li, Fang
Wu, Dongna
Issue Date
2014-08-15
Type
Article
Language
en_US
Keywords
Paris Saponin VII (PS VII) , Cervical Cancer , Proliferation , Apoptosis
Alternative Title
Abstract
Background:
Saponins of several herbs are known to induce apoptosis in many cancer cells. The present study aimed to investigate the growth inhibitory effect of Paris saponin VII (PS VII), a kind of steroidal saponins from Chonglou (Rhizoma Paridis Chonglou), on the human cervical cancer cell line Hela and the relative molecular mechanisms.
Methods:
Hela cells were exposed to different concentrations of PS VII (1 to 100 μM). Inhibition of cell proliferation was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 5-ethynyl-2′-deoxyuridine (EdU) assays. The amount of apoptotic cells was evaluated by flow cytometric analysis. And the protein level of cleaved caspase-3, cleaved caspase-9, Bax, and Bcl-2 was evaluated by Western blot.
Results:
The half maximal inhibitory concentration (IC50) value of PS VII for the growth inhibition of Hela cells was 2.62 ± 0.11 μM. PS VII increased the expression of caspase-3, caspase-9, and Bax while decreased that of Bcl-2, suggesting that PS VII may induce apoptosis through intrinsic apoptotic ways.
Conclusions:
These data indicate that PS VII has the potential for the treatment of cervical cancer.
Description
Citation
Zhang, W., Zhang, D., Ma, X., Liu, Z., Li, F., & Wu, D. (2014). Paris saponin VII suppressed the growth of human cervical cancer Hela cells. European journal of medical research, 19(1), 41. https://doi.org/10.1186/2047-783X-19-41
Publisher
European Journal of Medical Research