KIF14 affects cell cycle arrest and cell viability in cervical cancer by regulating the p27Kip1 pathway
Loading...
Authors
Zhang, Jie
Buranjiang, Gulimire
Mutalifu, Zuohelaguli
Jin, Hua
Yao, Liyan
Issue Date
2022-04-19
Type
Article
Language
en_US
Keywords
Cervical Cancer , KIF14 , Cell Cycle Arrest , P27 , Degradation
Alternative Title
Abstract
Background:
Cervical cancer is a kind of malignant gynecological tumor. The first choice for treating cervical cancer is still a combination of surgery and chemoradiotherapy, but the 5-year survival rate remains poor. Therefore, researchers are trying to find new ways to diagnose and treat cervical cancer early.
Methods:
The expression level of KIF14 in cells and tissues was determined via qRT–PCR. The ability of the cells to proliferate, migrate, and invade was examined using CCK-8 assay kits, colony formation assays, and Transwell chambers. The expression levels of Cyclin D1, Cyclin B1, p21, and p27 were also detected using western blot assays.
Results:
The results suggested that p27 is a key regulatory factor in the KIF14-mediated regulation of the cell cycle. In addition, KIF14 knockdown promotes malignancy in cervical cancer cells by inhibiting p27 degradation, resulting in cell cycle arrest.
Conclusions:
KIF14 is an oncogene in cervical cancer, and knocking down KIF14 causes cell cycle arrest by inhibiting p27 degradation, thus affecting cell viability, proliferation, and migration. These results provide a potential therapeutic target for cervical cancer.
Description
Citation
Zhang, J., Buranjiang, G., Mutalifu, Z., Jin, H., & Yao, L. (2022). KIF14 affects cell cycle arrest and cell viability in cervical cancer by regulating the p27Kip1 pathway. World journal of surgical oncology, 20(1), 125. https://doi.org/10.1186/s12957-022-02585-3
Publisher
World Journal of Surgical Oncology