Induction of mitochondria-mediated apoptosis in ca ski human cervical cancer cells triggered by mollic acid arabinoside isolated from leea indica
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Authors
Wong, Yau Hsiung
Kadir, Habsah Abdul
Issue Date
2011-11-20
Type
Article
Language
en_US
Keywords
Mitochondria-Mediated Apoptosis , Ca Ski Human Cervical Cancer Cells , Mollic Acid Arabinoside , Leea Indica
Alternative Title
Abstract
Leea indica is a medicinal plant traditionally used to treat cancer. Through bioassay-guided approach, we isolated mollic acid arabinoside (MAA), for the first time from Leea indica. Here, we present the apoptosis-inducing effect of MAA on Ca Ski cervical cancer cells. Based on DAPI staining, MAA-treated cells manifested nuclear shrinkage, condensation, and fragmentation. We further confirmed the fragmentation of DNA using TUNEL assay. During early apoptosis, MAA caused the perturbation of plasma membrane through externalization of PS, followed by the formation of apoptotic blebs. Prior to these events, MAA triggered rapid dissipation of the mitochondrial membrane potential. In the upstream, MAA increased the expression of Bax, decreased the expression of Bcl-2, and augmented the Bax/Bcl-2 ratio. These findings suggested that MAA induced mitochondrial-mediated apoptosis in Ca Ski cells and thus provide the scientific explanation for the traditional application of this herbal medicine in cancer treatment.
Description
Citation
Wong, Y. H., & Abdul Kadir, H. (2012). Induction of Mitochondria-Mediated Apoptosis in Ca Ski Human Cervical Cancer Cells Triggered by Mollic Acid Arabinoside Isolated from Leea indica. Evidence-based complementary and alternative medicine : eCAM, 2012, 684740. https://doi.org/10.1155/2012/684740
Publisher
eCAM