miR-375 is upregulated in acquired paclitaxel resistance in cervical cancer
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Authors
Shen, Y.
Wang, P.
Li, Y.
Ye, F.
Wang, F.
Wan, X.
Cheng, X.
Lu, W.
Xie, X.
Issue Date
2013-06-18
Type
Article
Language
en_US
Keywords
miR-375 , Paclitaxel , Chemo-Resistance , Cervical Cancer
Alternative Title
Abstract
Background: Chemo-resistance is one of the key causal factors in cancer death and emerging evidences suggest that microRNAs (miRNAs) have critical roles in the regulation of chemo-sensitivity in cancers. Cervical cancer is one of the most common malignancies in women and insensitive to chemotherapy clinically.
Methods: The differentially expressed miRNAs in cervical squamous cell carcinoma tissues were screened by using a microarray platform (μParaflo Sanger miRBase release 13.0). The expression of miR-375 was determined by stem-loop RT–PCR using 23 clinical cervical cancer samples and 2 cervical cancer cell lines. We exogenously upregulated miR-375 expression in SiHa and Caski cells using a pre-miRNA lentiviral vector transfection and observed its impact on paclitaxel sensitivity using MTS. The cells that stably overexpressed miR-375 were subcutaneously injected into mice to determine tumour growth and chemo-sensitivity in vivo.
Results: Twenty-one differentially expressed miRNAs were found by miRNA microarray between pro- and post-paclitaxel cervical cancer tissues. Of those, miR-375 showed consistent high expression levels across paclitaxel-treated cervical cells and tissues. Paclitaxel induced upregulated miR-375 expression in a clear dose-dependent manner. Forced overexpression of miR-375 in cervical cancer cells decreased paclitaxel sensitivity in vitro and in vivo.
Conclusion: Collectively, our results suggest that miR-375 might be a therapeutic target in paclitaxel-resistant cervical cancer.
Description
Citation
Shen, Y., Wang, P., Li, Y., Ye, F., Wang, F., Wan, X., Cheng, X., Lu, W., & Xie, X. (2013). miR-375 is upregulated in acquired paclitaxel resistance in cervical cancer. British journal of cancer, 109(1), 92–99. https://doi.org/10.1038/bjc.2013.308
Publisher
British Journal of Cancer