Tim-3 expression in cervical cancer promotes tumor metastasis
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Authors
Cao, Yang
Zhou, Xiaoxi
Huang, Xiaoyuan
Li, Qinlu
Gao, Lili
Jiang, Lijun
Huang, Mei
Zhou, Jianfeng
Issue Date
2013-01-15
Type
Article
Language
en_US
Keywords
Tim-3 Expression , Cervical Cancer , Tumor Metastasis
Alternative Title
Abstract
Background:
T cell immunoglobulin mucin-3 (Tim-3) has been identified as a negative regulator of anti-tumor immunity. Recent studies highlight the important role of Tim-3 in the CD8+ T cell exhaustion that takes place in both human and animal cancer models. However, the nature of Tim-3 expression in the tumor cell and the mechanism by which it inhibits anti-tumor immunity are unclear. This present study aims to determine Tim-3 is expressed in cervical cancer cells and to evaluate the role of Tim-3 in cervical cancer progression.
Methodology:
A total of 85 cervical tissue specimens including 43 human cervical cancer, 22 cervical intraepithelial neoplasia (CIN) and 20 chronic cervicitis were involved. Tim-3 expression in tumor cells was detected and was found to correlate with clinicopathological parameters. Meanwhile, expression of Tim-3 was assessed by RT-PCR, Western Blot and confocal microscopy in cervical cancer cell lines, HeLa and SiHa. The migration and invasion potential of Hela cells was evaluated after inhibiting Tim-3 expression by ADV-antisense Tim-3.
Conclusions:
We found that Tim-3 was expressed at a higher level in the clinical cervical cancer cells compared to the CIN and chronic cervicitis controls. We supported this finding by confirming the presence of Tim-3 mRNA and protein in the cervical cell lines. Tim-3 expression in tumor cells correlated with clinicopathological parameters. Patients with high expression of Tim-3 had a significant metastatic potential, advanced cancer grades and shorter overall survival than those with lower expression. Multivariate analysis showed that Tim-3 expression was an independent factor for predicting the prognosis of cervical cancer. Significantly, down-regulating the expression of Tim-3 protein inhibited migration and invasion of Hela cells. Our study suggests that the expression of Tim-3 in tumor cells may be an independent prognostic factor for patients with cervical cancer. Moreover, Tim-3 expression may promote metastatic potential in cervical cancers.
Description
Citation
Cao, Y., Zhou, X., Huang, X., Li, Q., Gao, L., Jiang, L., Huang, M., & Zhou, J. (2013). Tim-3 expression in cervical cancer promotes tumor metastasis. PloS one, 8(1), e53834. https://doi.org/10.1371/journal.pone.0053834
Publisher
PloS One