TLR4 and SARM1 modulate survival and chemoresistance in an HPV-positive cervical cancer cell line
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Authors
Morale, Mirian Galliote
Tamura, Rodrigo Esaki
Cintra, Ricardo
Araújo, Natália Meneses
Villa, Luisa Lina
Issue Date
2022-04-25
Type
Article
Language
en_US
Keywords
Cervical Cancer , Pattern Recognition Receptors
Alternative Title
Abstract
Human Papillomavirus is responsible for a wide range of mucosal lesions and tumors. The immune system participate in tumorigenesis in different ways. For example, signaling pathways triggered by Toll-like receptors (TLR) play a role in chemotherapy resistance in several tumor types and are candidates for contributing to the development of HPV-induced tumors. Here, we studied the receptor TLR4 and the adaptor molecule SARM1 in HeLa cells, an HPV-positive cervical cancer cell line. Knocking out of these genes individually proved to be important for maintaining cell viability and proliferation. TLR4 knock out cells were more sensitive to cisplatin treatment, which was illustrated by an increased frequency of apoptotic cells. Furthermore, TLR4 and SARM1 modulated ROS production, which was induced by cell death in response to cisplatin. In conclusion, TLR4 and SARM1 are important for therapy resistance and cervical cancer cell viability and may be relevant clinical targets.
Description
Citation
Morale, M. G., Tamura, R. E., Cintra, R., Araújo, N. M., & Villa, L. L. (2022). TLR4 and SARM1 modulate survival and chemoresistance in an HPV-positive cervical cancer cell line. Scientific reports, 12(1), 6714. https://doi.org/10.1038/s41598-022-09980-6
Publisher
Scientific Reports