Regulation of paclitaxel-induced programmed cell death by autophagic induction: a model for cervical cancer
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Authors
Chi, Eun Young
Viriyapak, Boonlert
Kwack, Hyun Sung
Lee, Yoon Kyung
Kim, Sang Il
Lee, Keun Ho
Park, Tae Churl
Issue Date
2013-03-12
Type
Article
Language
en_US
Keywords
Apoptosis , Autophagy , Cervical Neoplasms , mTOR Protein , Paclitaxel
Alternative Title
Abstract
Objective:
Autophagy plays a vital role in homeostasis by combining organelles and cellular proteins with lysosome under starvation conditions. In addition, autophagy provides tumor cells with a source of energy. Continued autophagy will induce cells death. Here we aim to see if autophagic induction has an effect on conventional chemotherapeutic agents.
Methods:
Rapamycin, or mammalian target of rapamycin and paclitaxel, apoptosis-inducing agents were used autophagy in HeLa cervical cancer cells.
Results:
Growth inhibition of cells was not observed after the application of 0, 10, 20 nM of paclitaxel with or without rapamycin. Using a 5 nM concentration of paclitaxel, rapamycin administration inhibited cell growth significantly compared to no treatment. This implies the synergic antitumor effect of paclitaxel and rapamycin. Paclitaxel itself did not show any autophagic effect on cells but did show cell apoptosis by flow cytometry. Light chain 3, a microtubule-associated protein, which reflect autophagy, was increased with 5 nM of paclitaxel after pretreatment with 10 nM of rapamycin.
Conclusion:
These findings suggest that the autophagic inducer, rapamycin, can potentiate autophagic cell death when added as an apoptosis-inducing chemotherapeutic agent. In conclusion, the control of autophagy may be a future target for chemotherapy.
Description
Citation
Chi, E. Y., Viriyapak, B., Kwack, H. S., Lee, Y. K., Kim, S. I., Lee, K. H., & Park, T. C. (2013). Regulation of paclitaxel-induced programmed cell death by autophagic induction: A model for cervical cancer. Obstetrics & gynecology science, 56(2), 84–92. https://doi.org/10.5468/OGS.2013.56.2.84
Publisher
Obstetrics & Gynecology Science