Picosecond pulsed electric fields induce apoptosis in a cervical cancer xenograft
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Authors
JIA, JIA
XIONG, ZHENG-AI
QIN, QIN
YAO, CHEN-GUO
ZHAO, XIAO-ZHEN
Issue Date
2014-11-14
Type
Article
Language
en_US
Keywords
Picosecond Pulsed Electric Fields , Human Cervical Carcinoma , Nude Mouse Xenograft , Apoptosis , Caspase
Alternative Title
Abstract
The aim of the present study was to evaluate the efficacy of picosecond pulsed electric fields (psPEF) on a cervical cancer xenograft. Human cervical cancer xenografts were established in nude mice by transplantation of HeLa cells, and the tumors were then treated with psPEF. The histological changes were observed by hematoxylin-eosin staining and transmission electron microscopy. The rate of tumor cell apoptosis was determined using a terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling assay. The mitochondrial transmembrane potential of the tumor cells was detected by laser scanning confocal microscopy, and the activity of caspase-3, -8, -9 and -12 was determined. The inhibitory rate seven days post-psPEF treatment was also calculated. The results showed that exposure to psPEF led to an increased rate of apoptosis, collapse of mitochondrial transmembrane potential, and activation of caspases. The inhibitory rate was 9.11% at day 7. The results of the present study indicate that psPEF may induce apoptosis in a cervical cancer xenograft through the endoplasmic reticulum stress and caspase-dependent signaling pathways.
Description
Citation
Jia, J., Xiong, Z. A., Qin, Q., Yao, C. G., & Zhao, X. Z. (2015). Picosecond pulsed electric fields induce apoptosis in a cervical cancer xenograft. Molecular medicine reports, 11(3), 1623–1628. https://doi.org/10.3892/mmr.2014.2953
Publisher
Molecular Medicine Reports