Morphine stimulates cervical cancer cells and alleviates cytotoxicity of chemotherapeutic drugs via opioid receptor‐dependent and ‐independent mechanisms
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Authors
Yu, Zhengwen
Jin, Sheng
Tian, Shiming
Wang, Zhibao
Issue Date
2022-10-06
Type
Article
Language
en_US
Keywords
Cervical Cancer , EGFR , Morphine , Opioid Receptor , RhoA
Alternative Title
Abstract
Morphine is frequently applied in cancer patients for pain management. However, its effects on cancer are not well understood but observed to be specific to certain cancer types. We previously revealed the stimulatory properties of morphine in esophageal carcinoma. This work addressed the effects of morphine and its underlying mechanisms in cervical cancer. Proliferation, apoptosis, and migration assays were performed to examine the effects of morphine alone and its combinatory effects with chemotherapeutic drugs. Immunoblotting and biochemical analysis were performed to determine the underlying mechanisms of morphine's action. Morphine promoted proliferation in opioid receptor‐dependent manner and stimulated migration in opioid receptor‐independent manner. However, morphine did not affect cervical cancer cell survival. Morphine also interfered with all tested chemotherapeutic drugs (e.g., cisplatin, 5‐FU, and paclitaxel) and alleviates their efficacy. Mechanistically, morphine‐stimulated growth via activating EGFR‐mediated signaling pathways and is opioid‐receptor‐dependent; morphine‐stimulated migration via activating RhoA‐mediated signaling pathways and this is opioid receptor‐independent. Our work suggests a strong correlation of this opioid receptor on growth factor signaling to stimulate growth and opioid receptor‐independent activation of RhoA and consequent migration. Our findings have the potential to guide the clinical use of morphine for patients with cervical cancer.
Description
Citation
Yu, Z., Jin, S., Tian, S., & Wang, Z. (2022). Morphine stimulates cervical cancer cells and alleviates cytotoxicity of chemotherapeutic drugs via opioid receptor-dependent and -independent mechanisms. Pharmacology research & perspectives, 10(5), e01016. https://doi.org/10.1002/prp2.1016
Publisher
Pharmacology Research & Perspectives