Tumour-associated CD66b+ neutrophil count is an independent prognostic factor for recurrence in localised cervical cancer
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Authors
Carus, A.
Ladekarl, M.
Hager, H.
Nedergaard, B. S.
Donskov, F.
Issue Date
2013-04-16
Type
Article
Language
en_US
Keywords
Tumour-Associated Neutrophils , Tumour-Associated Macrophages , Stereology , Cancer Inflammation
Alternative Title
Abstract
Background: The prognostic impact of tumour-promoting immune cells in cervical cancer is unclear.
Methods: Federation of Gynaecology and Obstetrics (FIGO) stage IB and IIA cervical cancer patients (N=101) were assessed for tumour-associated CD66b+ neutrophils and CD163+ macrophages by immunohistochemistry in whole tissue sections using stereology. Results were correlated with previous results on tumour-infiltrating CD3+, CD4+, and CD8+ lymphocytes in the same cohort with recurrence-free survival (RFS) as end point.
Results: The highest densities of CD66b+ neutrophils and CD163+ macrophages were observed in the peritumoural compartment (median 53.1 cells mm−2 and 1.3% area fraction, respectively). Above median peritumoural and stromal CD66b+ neutrophils and peritumoural CD163+ macrophages were significantly associated with short RFS. Multivariate analysis identified high peritumoural neutrophils (HR 2.27; 95% CI 1.09–4.75; P=0.03), low peritumoural CD8+ lymphocytes (HR 3.67; 95% CI 1.63–8.25; P=0.002), and lymph node metastases (HR 2.70; 95% CI 1.26–5.76; P=0.01) as independent prognostic factors for short RFS, whereas CD163+ macrophages were not significant. An index of combined intratumoral and peritumoral CD66b+ neutrophils to CD8+ lymphocytes had good discriminatory power for each quartile with 5-year RFS of 92%, 80%, 62%, and 44% (P=0.001).
Conclusion: Tumour-associated neutrophil count is an independent prognostic factor for short RFS in localised cervical cancer. Combining CD66b and CD8 may further improve prognostic stratification. These findings require prospective validation.
Description
Citation
Carus, A., Ladekarl, M., Hager, H., Nedergaard, B. S., & Donskov, F. (2013). Tumour-associated CD66b+ neutrophil count is an independent prognostic factor for recurrence in localised cervical cancer. British journal of cancer, 108(10), 2116–2122. https://doi.org/10.1038/bjc.2013.167
Publisher
British Journal of Cancer