m6A-related lncRNAs are potential prognostic biomarkers of cervical cancer and affect immune infiltration
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Authors
Jia, Haixia
Hao, Suhua
Cao, Meiting
Wang, Lifang
Bai, Hua
Shui, Wen
Yang, Xiaotang
Issue Date
2022-04-11
Type
Article
Language
en_US
Keywords
Prognostic Biomarkers , m6A-Related lncRNAs , Cervical Cancer , Immune Infiltration
Alternative Title
Abstract
The correlation of m6A-related lncRNAs with the prognosis and immune microenvironment of cervical cancer is not yet clear. In this study, we identified 7 m6A-related prognostic lncRNAs by Pearson correlation and univariate Cox regression analyses based on TCGA-cervical cancer dataset. Then, patients were divided into two clusters by consensus clustering based on the 7 m6A-related prognostic lncRNA expression. Cluster 1 was characterized by survival and stage disadvantage, enrichment of immunosuppressive and carcinogenic activation pathways. Besides, cluster 1 had higher immunosuppressive factor TGFbeta and lower immune cell infiltration compared with cluster 2. According to the expression of 7 m6A-related lncRNA, a 6-m6A-related lncRNA risk score model was established in the training set by LASSO regression analysis. The high-risk group had worse overall survival than the low-risk group. No matter in the training or validation sets, the m6A-related lncRNA risk score was an independent prognostic factor for overall survival. Meanwhile, we validated the independent prognostic value of risk score in the disease-specific survival and progression-free survival by multivariate Cox analysis. The high-risk group was characterized by higher TGFbeta and regulatory T cell and was rich in malignant pathways. Additionally, we also detected and compared the expression levels of four m6A-related prognostic lncRNA in 9 tumor samples and 9 normal tissues using quantitative real-time polymerase chain reaction assay. In conclusion, the novel m6A-related lncRNA risk score is a potential prognostic predictor of cervical cancer patients. These 6 m6A-related lncRNAs might serve as key mediators of the immune microenvironment and represent promising therapeutic targets for improving cervical cancer prognosis.
Description
Citation
Jia, H., Hao, S., Cao, M., Wang, L., Bai, H., Shui, W., & Yang, X. (2022). m6A-Related lncRNAs Are Potential Prognostic Biomarkers of Cervical Cancer and Affect Immune Infiltration. Disease markers, 2022, 8700372. https://doi.org/10.1155/2022/8700372
Publisher
Disease Markers