Long non-coding RNAs PGM5-AS1 upregulates Decorin (DCN) to inhibit cervical cancer progression by sponging miR-4284
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Authors
Wang, Huimin
Wang, Dan
Wei, Qiong
Li, Chun
Li, Chunyan
Yang, Jing
Issue Date
2022-04-14
Type
Article
Language
en_US
Keywords
lncRNA PGM5-AS1 , Cervical Cancer , miR-4284 , DCN
Alternative Title
Abstract
Long non-coding RNAs (lncRNAs) have been widely studied and play crucial roles in cervical cancer (CC) progression. Here, we investigated the function and mechanism of lncRNA PGM5-AS1 action in CC cells. Using real-time quantitative polymerase chain reaction or western blotting, PGM5-AS1 and decorin (DCN) were downregulated in CC tissues and cells, whereas miR-4284 was upregulated. Luciferase assay, RNA pull-down assay, and western blotting showed that PGM5-AS1 could sponge miR-4284 to upregulate DCN expression in CC cells. Additionally, cell functional experiments showed that PGM5-AS1 overexpression led to decreased proliferation, migration, and invasion of CC cells. However, the inhibitory effect of PGM5-AS1 overexpression on CC cells was partly relieved by DCN knockdown because of the targeting interaction between PGM5-AS1, miR-4284, and DCN. In summary, this study identified that PGM5-AS1 negatively regulates CC cell malignancy by targeting miR-4284/DCN.
Description
Citation
Wang, H., Wang, D., Wei, Q., Li, C., Li, C., & Yang, J. (2022). Long non-coding RNAs PGM5-AS1 upregulates Decorin (DCN) to inhibit cervical cancer progression by sponging miR-4284. Bioengineered, 13(4), 9872–9884. https://doi.org/10.1080/21655979.2022.2062088
Publisher
Bioengineered