Overview of microarray analysis of gene expression and its applications to cervical cancer investigation
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Authors
Chao, Angel
Wang, Tzu-Hao
Lai, Chyong-Huey
Issue Date
2008-08-15
Type
Article
Language
en_US
Keywords
Cervix , Gene Expression , Microarray , Neoplasm
Alternative Title
Abstract
Cervical cancer is one of the leading female cancers in Taiwan and ranks as the fifth cause of cancer death in the female population. Human papillomavirus has been established as the causative agent for cervical neoplasia and cervical cancer. However, the tumor biology involved in the prognoses of different cell types in early cancers and tumor responses to radiation in advanced cancers remain largely unknown. The introduction of microarray technologies in the 1990s has provided genome-wide strategies for searching tens of thousands of genes simultaneously. In this review, we first summarize the two types of microarrays: oligonucleotides microarray and cDNA microarray. Then, we review the studies of functional genomics in cervical cancer. Gene expression studies that involved cervical cancer cell lines, cervical cells of cancer versus normal ectocervix, cancer tissues of different histology, radioresistant versus radiosensitive patients, and the combinatorial gene expression associated with chromosomal amplifications are discussed. In particular, CEACAM5, TACSTD1, S100P, and MSLN have shown to be upregulated in adenocarcinoma, and increased expression levels of CEACAM5 and TACSTD1 were significantly correlated with poorer patient outcomes. On the other hand, 35 genes, including apoptotic genes (e.g. BIK, TEGT, SSI-3), hypoxia-inducible genes (e.g. HIF1A, CA12), and tumor cell invasion and metastasis genes (e.g. CTSL, CTSB, PLAU, CD44), have been noted to echo the hypothesis that increased tumor hypoxia leads to radiation resistance in cervical cancer during radiation.
Description
Citation
Chao, A., Wang, T. H., & Lai, C. H. (2007). Overview of microarray analysis of gene expression and its applications to cervical cancer investigation. Taiwanese journal of obstetrics & gynecology, 46(4), 363–373. https://doi.org/10.1016/S1028-4559(08)60005-4
Publisher
Taiwanese Journal of Obstetrics & Gynecology