CircCASC15-miR-100-mTOR may influence the cervical cancer radioresistance
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Authors
Yao, Tingting
Yao, Yao
Chen, Zhiliao
Peng, Yongpai
Zhong, Guanglei
Huang, Chunxian
Li, Jing
Li, Ruixin
Issue Date
2022-04-27
Type
Article
Language
en_US
Keywords
Cervical Cancer , Radio-Resistance , miR-100 , BSP , circCASC15
Alternative Title
Abstract
Background:
Cervical cancer has ranked the top one in gynecological malignancies for incidence. Radioresistance is now becoming a leading reason of recurrence.
Methods:
Our microRNA array data indicated that the miRNA-100 level decreased significantly during radioresistance. In this study, we up-regulated miR-100 in Hela and Siha cells by using miR-100 mimics and observed proliferation and invasion.
Results:
It turned out that with overexpression of miR-100, the cells had less invasiveness as well as proliferation. It may target gene mTOR, and it deed reduced EMT. To examine the role of miR-100 in radioresistance, there was no significant result showed by BSP. While the circCASC15 has been identified with sponge function according to RNA pull down and ISH.
Conclusion:
The conclusions indicate miR-100 is a tumor suppressor gene and could be a therapeutic target in radio-resistant cervical cancers.
Description
Citation
Yao, T., Yao, Y., Chen, Z., Peng, Y., Zhong, G., Huang, C., Li, J., & Li, R. (2022). CircCASC15-miR-100-mTOR may influence the cervical cancer radioresistance. Cancer cell international, 22(1), 165. https://doi.org/10.1186/s12935-022-02573-3
Publisher
Cancer Cell International