Effect of Exosomal lncRNA MALAT1/miR-370-3p/STAT3 positive feedback loop on PI3K/Akt pathway mediating cisplatin resistance in cervical cancer cells
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Authors
Hu, Yi
Li, Genlin
Ma, Yan
Luo, Guifang
Wang, Qian
Zhang, Shufen
Issue Date
2023-02-06
Type
Article
Language
en_US
Keywords
Exosomal lncRNA , MALAT1/miR-370-3p/STAT3 , PI3K/Akt Pathway , Cisplatin Resistance , Cervical Cancer Cells
Alternative Title
Abstract
Background:
Exosomes can encapsulate lncRNA to mediate intercellular communication in cancer progression. Our study devoted to research the effect that long noncoding RNA Metastasis-associated lung adenocarcinoma transcript 1 (lncRNA MALAT1) influence on cervical cancer (CC).
Methods:
MALAT1 and miR-370-3p levels in CC was assessed using qRT-PCR. CCK-8 assay and flow cytometry were devoted to confirm the influence on MALAT1 influencing the proliferation in cisplatin-resistant CC cells. Futher more, MALAT1, combined with miR-370-3p was confirmed by dual-luciferase reporter assay and RNA immunoprecipitation assay.
Results:
In CC tissues, MALAT1 turned into substantially expressed, cisplatin-resistant cell lines, as well as exosomes. Cell proliferation was restrained and cisplatin-induced apoptosis was promoted by way of Knockout MALAT1. And promoted the miR-370-3p level, MALAT1 targeted miR-370-3p. Promoting effect of MALAT1 on cisplatin resistance of CC was partially reversed through miR-370-3p. In addition, STAT3 may induce up-regulation of MALAT1 expression in cisplatin-resistant CC cells. It was further confirmed that the effect of MALAT1 on cisplatin-resistant CC cells was achieved by activating PI3K/Akt pathway.
Conclusion:
The positive feedback loop of exosomal MALAT1/miR-370-3p/STAT3 mediates the cisplatin resistance of cervical cancer cells affecting PI3K/Akt pathway. Exosomal MALAT1 may become a promising therapeutic target for treating cervical cancer.
Description
Citation
Hu, Y., Li, G., Ma, Y., Luo, G., Wang, Q., & Zhang, S. (2023). Effect of Exosomal lncRNA MALAT1/miR-370-3p/STAT3 Positive Feedback Loop on PI3K/Akt Pathway Mediating Cisplatin Resistance in Cervical Cancer Cells. Journal of oncology, 2023, 6341011. https://doi.org/10.1155/2023/6341011
Publisher
Journal of Oncology