Identification of protein biomarkers for cervical cancer using human cervicovaginal fluid

dc.contributor.authorRaemdonck, Geert A. A. Van
dc.contributor.authorTjalma, Wiebren A. A.
dc.contributor.authorCoen, Edmond P.
dc.contributor.authorDepuydt, Christophe E.
dc.contributor.authorOstade, Xaveer W. M. Van
dc.date.accessioned2023-01-17T17:26:58Z
dc.date.available2023-01-17T17:26:58Z
dc.date.issued2014-09-12
dc.description.abstractObjectives: Cervicovaginal fluid (CVF) can be considered as a potential source of biomarkers for diseases of the lower female reproductive tract. The fluid can easily be collected, thereby offering new opportunities such as the development of self tests. Our objective was to identify a CVF protein biomarker for cervical cancer or its precancerous state. Methods: A differential proteomics study was set up using CVF samples from healthy and precancerous women. Label-free spectral counting was applied to quantify protein abundances. Results: The proteome analysis revealed 16 candidate biomarkers of which alpha-actinin-4 (p = 0.001) and pyruvate kinase isozyme M1/M2 (p = 0.014) were most promising. Verification of alpha-actinin-4 by ELISA (n = 28) showed that this candidate biomarker discriminated between samples from healthy and both low-risk and high-risk HPV-infected women (p = 0.009). Additional analysis of longitudinal samples (n = 29) showed that alpha-actinin-4 levels correlated with virus persistence and clearing, with a discrimination of approximately 18 pg/ml. Conclusions: Our results show that CVF is an excellent source of protein biomarkers for detection of lower female genital tract pathologies and that alpha-actinin-4 derived from CVF is a promising candidate biomarker for the precancerous state of cervical cancer. Further studies regarding sensitivity and specificity of this biomarker will demonstrate its utility for improving current screening programs and/or its use for a cervical cancer self-diagnosis test.en_US
dc.identifier.citationVan Raemdonck, G. A., Tjalma, W. A., Coen, E. P., Depuydt, C. E., & Van Ostade, X. W. (2014). Identification of protein biomarkers for cervical cancer using human cervicovaginal fluid. PloS one, 9(9), e106488. https://doi.org/10.1371/journal.pone.0106488en_US
dc.identifier.otherDOI: 10.1371/journal.pone.0106488
dc.identifier.urihttps://hdl.handle.net/20.500.14041/5621
dc.language.isoen_USen_US
dc.publisherPloS Oneen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.subjectProtein Biomarkersen_US
dc.subjectCervical Canceren_US
dc.subjectHuman Cervicovaginal Fluiden_US
dc.titleIdentification of protein biomarkers for cervical cancer using human cervicovaginal fluiden_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
pone.0106488.pdf
Size:
1.19 MB
Format:
Adobe Portable Document Format
Description:
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.65 KB
Format:
Item-specific license agreed upon to submission
Description: