circ_ZFR is linked to paclitaxel resistance in cervical cancer via mir-944 sponging and IL-10 upregulation
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Authors
Long, Xiaoqian
Zheng, Meiyun
Yang, Youlin
Chen, Yi
Zhang, Xiahui
Zhang, Haiyan
Issue Date
2022-01-27
Type
Article
Language
en_US
Keywords
circ_ZFR , Paclitaxel Resistance , Cervical Cancer , miR-944 , IL-10 Upregulation
Alternative Title
Abstract
Objective:
Cervical cancer (CC) has an elevated rate of invasion and death despite surgical treatment, radiotherapy, and chemotherapy. Several studies revealed that circRNAs have a key contribution to the resistance of drugs against different types of carcinomas. The goal of the existing study was to figure out what role circ_ZFR plays in paclitaxel (PTX) resistance in cervical cancer (CC) patients.
Materials and Methods:
Herein, two types of CC cells (SiHa/PTX and Hela/PTX) were utilized. The levels of IL-10 mRNA, miR-944, and circ_ZFR were measured using qRT-PCR analyses. The CCK-8 assay was used to determine PTX resistance. The IL-10 expression was measured via the ELISA technique. The combination of miR-944 and circ_ZFR or IL-10 was validated using a dual-luciferase reporter (DLR) assay.
Results:
The amount of circ_ZFR was increased in PTX-resistant CC cells and tissues. In PTX-resistant CC cells, knocking down circ_ZFR expression decreased PTX resistance. circ_ZFR knockdown significantly reduced IL-10 expression via sponging miR-944, increasing PTX sensitivity in PTX-resistant CC cells.
Conclusion:
circ_ZFR knockdown has a considerable role in overwhelming CC-associated PTX resistance by modifying the axis of miR-944/IL-10 axis, suggesting that developing a circRNA target-based treatment could be considered prevent CC progression.
Description
Citation
Long, X., Zheng, M., Yang, Y., Chen, Y., Zhang, X., & Zhang, H. (2022). circ_ZFR Is Linked to Paclitaxel Resistance in Cervical Cancer via miR-944 Sponging and IL-10 Upregulation. Analytical cellular pathology (Amsterdam), 2022, 4807287. https://doi.org/10.1155/2022/4807287
Publisher
Analytical Cellular Pathology (Amsterdam)