Analysis of DNA variants in miRNAs and miRNA 3’UTR binding sites in female infertility patients
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Authors
yc, Katarzyna M. T
Wong, Anthony
Scott, Richard T. Jr.
Tao, Xin
Schindler, Karen
Xing, Jinchuan
Issue Date
2020-10-17
Type
Article
Language
en_US
Keywords
Female Infertility , miRNA , Early Embryogenesis , Maternal-to-zygotic Transition
Alternative Title
Abstract
Early human embryogenesis relies on maternal gene products accumulated during oocyte growth and maturation, until around day-3 post-fertilization when human zygotic genome activation occurs. The maternal-to-zygotic transition (MZT) is a tightly coordinated process of selective maternal transcript clearance and new zygotic transcript production. If MZT is disrupted, it will lead to developmental arrest and pregnancy loss. It is well established that microRNA (miRNA) mutations disrupt regulation of their target transcripts. We hypothesize that some cases of embryonic arrest and pregnancy loss could be explained by the mutations in the maternal genome that affect miRNA-target transcript pairs. To this end, we examined mutations within miRNAs or miRNA binding sites in the 3’ untranslated regions (3’UTR) of target transcripts. Using whole exome sequencing data from 178 women undergoing in vitro fertilization (IVF) procedures, we identified 1,197 variants in miRNA genes, including 93 single nucleotide variants (SNVs) and 19 small insertions/deletions (INDELs) within the seed region of 100 miRNAs. Eight miRNA seed-region variants were significantly enriched among our patients when compared to a normal population. Within predicted 3’UTR miRNA binding sites, we identified 7,393 SNVs and 1,488 INDELs. Between our patients and a normal population, 52 SNVs and 30 INDELs showed significant association in the single variant testing, whereas 51 genes showed significant association in the gene-burden analysis for genes that are expressed in preimplantation embryos. Interestingly, we found that many genes with disrupted 3’UTR miRNA binding sites follow gene expression patterns resembling MZT. In addition, some of these variants showed dramatic allele frequency difference between the patient and the normal group, offering potential utility as biomarkers for screening patients prior to IVF procedures.
Description
Citation
Tyc, K. M., Wong, A., Scott, R. T., Jr, Tao, X., Schindler, K., & Xing, J. (2021). Analysis of DNA variants in miRNAs and miRNA 3'UTR binding sites in female infertility patients. Laboratory investigation; a journal of technical methods and pathology, 101(4), 503–512. https://doi.org/10.1038/s41374-020-00498-x
Publisher
A Journal of Technical Methods and Pathology