Tissue and sperm handling before assisted reproductive technology (ART): A systematic review
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Authors
Ambar, Rafael Favero
Gava, Marcello M
Ghirelli-Filho, Milton
Yoshida, Ivan H
De Paula, Thais Serzedello
Glina, Sidney
Issue Date
2021-07-22
Type
Article
Language
en_US
Keywords
Male Infertility , Assisted Reproduction , Azoospermia , Cryopreservation , Sperm Retrieval , Sperm Selection
Alternative Title
Abstract
Objective: : To explore the medical literature on techniques of tissue and sperm handling after surgical retrieval for intracytoplasmic sperm injection (ICSI).
Methods: : A search was performed in PubMed and Google Scholar databases, according to a modified Preferred Reporting Items for Systemic Reviews and Meta-Analyses (PRISMA) guideline, considering the studies investigating tissue handling and sperm selection techniques for ICSI.
Results: : Overall, 42 articles were included in this study, investigating sample handling, methods for sperm selection, and the use of chemical compounds to improve sperm motility and fertilisation rates.
Conclusion: : The ideal sperm handling method should provide a high sperm count, high vitality and appropriate sperm function, without side-effects. In this review the most common and useful techniques are described and the best combination strategies discussed in clinical scenarios.
Description
Citation
Ambar RF, Gava MM, Ghirelli-Filho M, Yoshida IH, De Paula TS, Glina S. Tissue and sperm handling before assisted reproductive technology (ART): A systematic review. Arab J Urol. 2021 Jul 22;19(3):238-246. doi: 10.1080/2090598X.2021.1954801. PMID: 34552775; PMCID: PMC8451624.
Publisher
Arab journal of urology