Genetics of female infertility: molecular study of newborn ovary homeobox gene in poor ovarian responders

Batiha, Osamah; Alahmad, Nour Alhoda; Sindiani, Amer; Bodoor, Khaldon; Shaaban, Sherin; Al-Smadi, Mohammad

Genetics of female infertility: molecular study of newborn ovary homeobox gene in poor ovarian responders

Files

JHRS-12-85.pdf (490.1 KB)

Authors

Batiha, Osamah

Alahmad, Nour Alhoda

Sindiani, Amer

Bodoor, Khaldon

Shaaban, Sherin

Al-Smadi, Mohammad

Issue Date

2019-04

Type

Article

Language

en_US

Keywords

Female Infertility , Newborn Ovary Homeobox Gene , Poor Ovarian Response

Abstract

Background: Newborn ovary homeobox (NOBOX) gene plays a critical role in the transcriptional regulation of oocyte-specific genes. Previous studies have demonstrated a pathogenic effect of NOBOX variants on premature ovarian insufficiency (POI) patients. Poor ovarian response (POR) is a risk factor for POI. Therefore, genetic variants in the NOBOX gene may also be studied as risk factors for POR development. Aims: The aim of the study is to investigate the association between seven known NOBOX single-nucleotide polymorphisms (SNPs) and POR in Jordanian females. Settings and Design: This was a case–control study of 60 females with POR for controlled ovarian hyperstimulation and 59 healthy females with no history of reproductive problems. Blood samples were collected from the participants and seven SNPs of NOBOX gene were screened. Subjects and Methods: DNA was extracted from blood samples. Polymerase chain reaction with primers specific for seven known SNPs in NOBOX gene was used to amplify the specified region within the gene followed by Sanger sequencing. Results: The seven SNPs investigated in this study, namely, rs77587352 (c.271G>T, p. Gly91Trp), rs7800847 (c.349C>T, p. Arg117Trp), rs193303102 (c.907C>T, p. Arg303X), rs193303103 (c.1025G>C, p. Ser342Thr), rs193303104 (c.1048G>T, p. Val350Leu), rs201947677 (c.1064G>A, p. Arg355His), and rs146227301 (c.1856C>T, p. Pro619Leu), only represent the wild-type allele in both females with POR and healthy participants. Conclusions: The results show that only monomorphic genotype of the NOBOX variants was found in Jordanian females studied.

Citation

Batiha, O., Alahmad, N. A., Sindiani, A., Bodoor, K., Shaaban, S., & Al-Smadi, M. (2019). Genetics of Female Infertility: Molecular Study of Newborn Ovary Homeobox Gene in Poor Ovarian Responders. Journal of human reproductive sciences, 12(2), 85–91. https://doi.org/10.4103/jhrs.JHRS_112_18

Publisher

Journal of Human Reproductive Sciences